Composite

Part:BBa_K3332100:Design

Designed by: Shiyi Zhai   Group: iGEM20_XMU-China   (2020-10-18)


RNAi sequence of phnF-RNAi sequence of phnJ-J23100-RBS-phnJ-T-J23100-RBS-phnO-T-J23100-RBS-phnE1E2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 438
    Illegal NheI site found at 461
    Illegal NheI site found at 869
    Illegal NheI site found at 892
    Illegal NheI site found at 1921
    Illegal NotI site found at 1036
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2134
    Illegal XhoI site found at 281
    Illegal XhoI site found at 712
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1520
    Illegal NgoMIV site found at 1542
    Illegal AgeI site found at 4812
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1532


Design Notes

None


Source

Construct from existed parts according to Standard BioBrick Assembly

References